The Synapt HDMS (Waters) system is a quadrupole orthogonal time of flight mass spectrometer and also has a travelling wave ion mobility spectrometrydevice. The quadrupole has a mass range up to m/z 32 000 to enable MS/MS analysis of macromolecular biomolecules and non-covalent complexes. It has a NanoMate Triversa (Advion Biosciences) automated injection system and nanoESI interface.