A Laser excites the fluorescent dyes bound to the cells. The emitted light has a longer wavelength and passes through a bandpass filter to shut out the excitatory and other unwanted light. The strength of this fluorescent light is measured with a detector (PMT) for each cell and is displayed in form of a histogram.
For more information and specs:
http://www.ncl.ac.uk/fccf/equipment/
http://www2.le.ac.uk/departments/csmm/facilities/facs-aria-ii-flow-cytometer
Dr Andrew Filby | |
andrew.filby@ncl.ac.uk |
http://www.ncl.ac.uk/fccf/contact/ | |
fccf@newcastle.ac.uk |